Xenbase Image ID: 40737

Fig. 1. Mis-activating early pronephric Notch signalling caused ectopic medial pronephrogenesis. (A-J) X. laevis embryos were injected at the eight-cell stage into a V2 blastomere to target tissues including the presumptive pronephric region. notch-ICD mRNA or deltaSTU mRNA was co-injected with beta-galactosidase mRNA to act as a lineage tracer [blue (A,B); red (C-J) staining on the injected side]. At stage 41, 3G8 is ectopic (white arrows) and 4A6 is reduced in embryos injected with notch-ICD mRNA (A). deltaSTU reduces 3G8 staining (white arrow) but has no effect on 4A6 staining (B). At stage 36, Na+K+ATPase expression is reduced after overexpression of notch-ICD (C), but only the proximal domain of Na+K+ATPase expression is reduced upon deltaSTU overexpression (D). slc5a2 expression is inhibited after overexpression of notch-ICD (E) and deltaSTU (F). At stage 32, odf3 (white arrows, G) and nephrin (black arrow, I) expression is ectopic upon notch-ICD mRNA injection. odf3 (white circle, H) and nephrin (J) are inhibited by deltaSTU. Asterisk denotes injected side. Image published in: Naylor RW and Jones EA (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue atp1b1.S atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase X. laevis Throughout NF stage 33 and 34 early proximal tubule late proximal tubule early distal tubule late distal tubule slc5a2.L X. laevis Throughout NF stage 33 and 34 early proximal tubule late proximal tubule cimap1a.L odf3 X. laevis Throughout NF stage 33 and 34 epidermis pronephric nephrostome nphs1.S nephrin X. laevis Throughout NF stage 33 and 34 glomus Image source: Published Larger Image Printer Friendly View

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