Xenbase Image ID: 40741

Fig. 5. hrt1 is induced by notch-ICD mRNA injection and promotes wnt4 expression. (A-E) X. laevis embryos were injected as previously described and left to develop to stage 28, when in situ hybridisation detecting expression of either wnt4 (A-C) or hrt1 (D,E) was performed. Embryos injected with hormone-inducible hrt1-hGR mRNA, but not incubated in dexamethasone to activate the message, have normal pronephric wnt4 expression (A). Embryos injected with hrt1-hGR mRNA but incubated in dexamethasone from stage 18 onwards, display ectopic wnt4 expression (B). MO depletion of HRT-1 inhibited wnt4 expression (C). Overexpression of notch-ICD causes a large increase in pronephric hrt1 expression (D), whereas hrt1 expression is largely unaffected by wnt4 mRNA injection (E). Asterisk denotes injected side. Image published in: Naylor RW and Jones EA (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wnt4.L wnt-4, Xwnt-4, Xwnt4 X. laevis Throughout NF stage 28 pronephric mesenchyme hey1.S bc8, chf2, herp2, hesr1, hrt-1, hrt1, oaf1, XHRT1 X. laevis Throughout NF stage 28 pronephric mesenchyme glomeral mesenchyme Image source: Published Larger Image Printer Friendly View

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