Xenbase Image ID: 40842

Fig. 2. Expression of FoxO genes. (A) Temporal expression patterns of FoxO1, FoxO3, FoxO4 and FoxO6 genes during Xenopus embryogenesis and relative expression in adult tissues. Amount of transcripts was analyzed by semi-quantitative RT-PCR using RNA isolated from embryos at indicated developmental stages (Nieuwkoop and Faber, 1967) or from adult tissues. Expression levels are shown in relative units. Histone H4 was used as internal control. (B-M) Spatial patterns analyzed by whole mount in situ hybridization of FoxO4 (B-H) and FoxO6 (I-M). (B) 2-cell stage, lateral view. (C) Stage 17, anterior view. (D) Stage 21, anterior view. (E) Stage 23 embryo hybridized with a FoxO4 sense probe, anterior view. (F) Magnification of the head of an embryo, stage 29. (G) Stage 35/36, lateral view. (H) Transversal section of the head, stage 38. (I) Stage 22, anterior view. (J) Stage 27, lateral view. (K) Magnification of the head at stage 28, anterior view. (L) Stage 32, lateral view. (M) Stage 30, anterior view. Red arrows indicate the eye field, yellow arrows indicate the olfactory pits. ba, branchial arches; hg, hatching gland; hm, head mesenchyme; l, lens; nt, neural tube; ov, otic vesicle; pd, pronephric duct; pl, presumptive lens; pr, presumptive retina; r, retina. Image published in: Schuff M et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxo4.L mllt7 X. laevis Throughout NF stage 17 neural plate foxo6.S xFox06 X. laevis Sometime during NF stage 27 to NF stage 28 hatching gland foxo4.L mllt7 X. laevis Throughout NF stage 21 neural crest optic field foxo4.L mllt7 X. laevis Throughout NF stage 29 and 30 head otic vesicle branchial arch retina lens placode foxo4.L mllt7 X. laevis Throughout NF stage 35 and 36 eye head pronephric kidney pronephric duct mesenchyme pharyngeal arch branchial arch foxo4.L mllt7 X. laevis Throughout NF stage 37 and 38 head mesenchyme retina lens neural tube roof plate foxo6.S xFox06 X. laevis Throughout NF stage 32 branchial arch head spinal cord foxo6.S xFox06 X. laevis Throughout NF stage 29 and 30 olfactory region branchial arch head olfactory pit Image source: Published Larger Image Printer Friendly View

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