Xenbase Image ID: 41187

Fig. 3. The sox2 expressed in β-catenin morphants is FGF-dependent. X. laevis embryos were injected with β-catenin MO and cultured in SU5402 to the stage indicated, then fixed and assayed for expression of xbra (A) or sox2 (B). (A) Control uninjected DMSO-treated embryos express xbra in the notochord and posterior mesoderm at stage 14, and in the posterior mesoderm only at stage 18. This expression is lost in embryos treated with the Fgf receptor inhibitor SU5402. β-catenin morphants treated with DMSO express xbra in a ring around the blastopore at stages 14 and 18, and this expression is also lost with SU5402 treatment. (B) Expression of sox2 in uninjected embryos is reduced by SU5402 treatment, although small amounts of sox2 expression are retained anteriorly for all doses of SU5402 at stage 14, and for all but the highest doses of SU5402 at stage 18. In β-catenin embryos, sox2 is expressed in a ring around the blastopore, which is very sensitive to SU5402 and is lost even at low SU5402 doses both at stage 14 and 18. Image published in: Wills AE et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 14 mesoderm axial mesoderm posterior sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 14 neural plate pre-chordal neural plate chordal neural plate tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 18 mesoderm posterior sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18   sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18 neural tube trigeminal placode Image source: Published Larger Image Printer Friendly View

Return to previous page