Xenbase Image ID: 41190

Fig. 6. Anterior neural markers are more sensitive to the loss of Bmp antagonists than posterior neural markers. X. laevis embryos were injected at the two-cell stage with increasing doses of Follisatin, Chordin, and Noggin MOs. Embryos were injected in both blastomeres at the two-cell stage; doses indicated refer to the total dose of MO injected into the embryo. Embryos were raised to stage 22, fixed and analyzed by in situ hybridization for expression of the genes indicated. nkx2.1 marks the ventral forebrain and is lost in embryos injected with low doses of FCN MOs. The forebrain/midbrain marker otx2, midbrain/hindbrain boundary marker en2 and rhombomeres 3/5 marker krox20 have reduced expression in embryos injected with low or intermediate doses of MO, and are lost in embryos injected with a high dose of MOs. The spinal cord marker hoxB9 is expressed strongly in embryos injected with low or intermediate doses of MO and is lost in embryos injected with a high dose of MO. Embryos are shown in lateral views with anterior to the left. Image published in: Wills AE et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 22 forebrain optic vesicle nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 22 forebrain olfactory bulb neuroectoderm en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 22 midbrain-hindbrain boundary egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 22 cranial neural crest rhombomere R3 rhombomere R5 hoxc9-like.L X. laevis Throughout NF stage 22 spinal cord posterior neural tube sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 22 optic vesicle brain forebrain midbrain hindbrain olfactory region spinal cord trigeminal ganglion Image source: Published Larger Image Printer Friendly View

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