Xenbase Image ID: 41191

Fig. 7. Xenopus ectodermal explants and ventralized embryos do not express neural border markers. Uninjected control X. laevis embryos, β-catenin morphants, and animal caps cut from blastula stage uninjected or noggin-injected embryos were cultured to stage 20 and assayed for expression of the neural border markers pax3, hairy2a, and zic2, and the neural crest marker slug. Expression of these genes was not detected in animal caps or β-catenin morphants (of β-catenin morphants, 0% expressed hairy2a (n = 14), pax3 (n = 15), and zic2 (n = 14), while 6% expressed slug weakly (n = 16). All four genes had some expression in animal caps cut from embryos injected with 1 pg of noggin mRNA (63% for hairy2a, n = 8; 50% for pax3, n = 10; 79% for slug, n = 14; and 67% for zic2, n = 9) . The epidermal marker cytokeratin is strongly expressed in β-catenin morphants but excluded from the blastopore. Cytokeratin is strongly expressed in both injected and uninjected animal caps, but was somewhat weaker in noggin-injected animal caps. Embryos are shown in dorsal views with anterior to the left. Image published in: Wills AE et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue zic2 hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 Xenopus Throughout NF stage 20 anterior neural fold neural crest pax3 cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 Xenopus Throughout NF stage 20 anterior neural fold forebrain midbrain hatching gland neural tube snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 Xenopus Throughout NF stage 20 neural crest neural tube anterior neural tube hes4 H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b Xenopus Throughout NF stage 20 anterior neural fold neural crest branchial crest hyoid crest mandibular crest krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 20 epidermis epidermis outer layer ectoderm non-neural ectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page