Xenbase Image ID: 41219

Fig. 5. Blocking Notch signaling impairs morphogenetic movements during gastrulation. (A) Wild type embryos of the same batch and derived from eggs of the same female were injected with 20 ng of Control Mo or with 20 ng of Notch Mo before the first mitotic division. The progress of the blastopore was recorded through gastrulation by scanning the vegetal sides at stages 10.25, 10.5, 11.5 and 12. Data are represented as the ratio between the perimeter of the blastopore and the embryo's perimeter in Notch Mo- (gray bars) and Control Mo- (white bars) injected embryos. Above each bar, an embryo representing the mean of the group is shown. For additional details, see legend to Table 4. This figure illustrates experiment No. 2. (B–E) Embryos of the same batch and derived from eggs of the same female were treated from the 1-cell stage with 100 μM of the γ-secretase inhibitor DAPT or with vehicle alone (DMSO) and fixed at stage 11 (B,C) for ISH of chd (turquoise staining) or at stage 14 for ISH of chd (turquoise staining) and N-tubulin (N-tub, purple staining). Image published in: Revinski DR et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 11 blastopore lip upper blastopore lip mesoderm tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin Xenopus Throughout NF stage 14 neuroectoderm anterior neural fold Image source: Published Larger Image Printer Friendly View

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