XB-IMG-41344
Xenbase Image ID: 41344
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Figure 4. Sox9 depletion results in the loss of gene expression in the dorsal aspect of the otic vesicle. A: The otic vesicle of embryos injected in one blastomere at the eight-cell stage with 2.5 ng of Sox9MO exhibited reduced Wnt3a and Pax2 expression associated with a dorsal expansion of Bmp4 and Sox2 at stage 35. The expression of Otx2 in the ventral aspect of the otic vesicle was also reduced but primarily along the antero-posterior axis. For whole-mount in situ hybridization, lateral views, dorsal to top. Anterior is to the right (control) or to the left (injected). Histology panels (dorsal to top) show transverse sections of Wnt3a- and Pax2-stained embryos. In the case of Bmp4-, Otx2-, and Sox2-stained embryos longitudinal sections were performed. The arrows point to the otic vesicle on the injected side B: A three dimensional representation of the otic vesicle at stage 35 illustrates the changes in expression pattern of Wnt3a, Pax2, Bmp4, Sox2, and Otx2 in injected as compared to controls otocysts. The position of the dorsoventral, anteroposterior, and mediolateral axis is indicated. D, dorsal; L, lateral; P, posterior. Scale bar = 200 mu m in A. Image published in: Park BY and Saint-Jeannet JP (2010) Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons.
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