Xenbase Image ID: 41351

Fig. 4. Somatic mutation of Wnt7b in macrophages prevents normal repair and regeneration of the kidney following ischemia reperfusion injury. (A and B) Genomic map and PCR products (tail or macrophage genomic DNA) showing the third exon of Wnt7b is deleted in monocytes/macrophages of mice with the LoxP-flanked conditional allele Wnt7bC3 and the transgene Csf1r-icre. WT allele, 153 bp; C3 allele, 200 bp; δC3 allele, no product. (C–E) PAS-stained sections of the outer cortex of Csf1R-icre; Wnt7bC3/− kidneys or control kidneys (Csf1R-icre; Wnt7bC3/+ and Wnt7bC3/−) 7d following injury. (F–J) Quantification of inflammation, injury, and repair parameters 7d postinjury of Csf1R-icre; Wnt7bC3/− or control mice (Csf1R-icre; Wnt7bC3/+ and Wnt7bC3/−). (K and L) Immunofluorescence images of kidneys showing dissolution of epithelial basement membrane (arrowheads) and quantification of dissolution in control and experimental (Csf1R-icre; Wnt7bC3/−) mice. (M–O) Immunofluorescence images of experimental postinjury kidneys showing the presence of markers of the cell cycle in epithelial cells and quantification of epithelial cells entering G1/S or in G2M phase of the cell cycle. P < 0.05. n = 5 or 6/group. (Scale bars, 50 μm.) Image published in: Lin SL et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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