Xenbase Image ID: 41520

Fig. 5. Xnr1 and Xnr2 act redundantly to control gastrulation but not mesendoderm induction. (A) Xenopus 4-cell embryos were injected in the two dorsal cells with 5 ng/cell of the indicated MOs, alone or in combination (2.5 ng each), together with 2.5 ng/cell FLDx. Embryos were fixed at stage 15 and processed for WISH and FLDx staining. Dorsal views, anterior to the top. Extending notochord (Xbra, Xnot-2) and prechordal mesoderm (goosecoid) were present but abnormally positioned. (B) DMZ was explanted at stage 10 from the same embryos as in A and cultured for 12 hours. For the purposes of rescue, 5 μg/ml recombinant Nodal protein was added to the culture medium after explantation. Elongation was significantly suppressed in double Xnr1/Xnr2 morphant explants, but was rescued by Nodal protein. (C) Head mesoderm was explanted at stage 10.25 from the same embryos as in A, plated on fibronectin-coated dishes and cultured for 3 hours. Spreading was severely impaired in double Xnr1/Xnr2 morphant explants, but was rescued in the presence of 5 μg/ml recombinant Nodal protein. Image published in: Luxardi G et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 15 axial mesoderm gsc goosecoid, gsc-a, gsc-b, Xgsc Xenopus Throughout NF stage 15 axial mesoderm Image source: Published Larger Image Printer Friendly View

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