Xenbase Image ID: 41521

Fig. 6. The Nodal pathway does not regulate the Wnt/PCP pathway. (A,B) Xenopus embryos were injected with 10 ng Lefty recombinant protein, treated with 200 μM SU5402 or 200 μM SB-505124 at the indicated stages, and analysed by WISH at stage 12 (A) or 14 (B). SU5402, but not SB-505124, repressed Xbra and Xwnt11. All views are vegetal. (C) Four-cell embryos were marginally injected with a mixture of 250 pg/blastomere dsh-GFP and 1 ng/blastomere mcherry mRNAs in the two dorsal cells. Stage 12 embryos were fixed, sectioned, stained with an anti-GFP antibody and imaged with an apotome microscope. (D) The percentage of cells that displayed Dsh-GFP fluorescence predominantly at the cell membrane among the mCherry-positive cells. Three embryos were analysed per condition, and the total number of cells analysed is indicated. *, P=0.0314. Image published in: Luxardi G et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wnt11 Wnt11-R, WNT11-related, wnt11a, Wnt11r Xenopus Throughout NF stage 12 mesoderm marginal zone circumblastoporal collar tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 12 mesoderm circumblastoporal collar marginal zone Image source: Published Larger Image Printer Friendly View

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