Xenbase Image ID: 41522

Fig. 7. Nodal signalling directly activates movement-effector genes. (A) Xenopus embryos were injected with 10 ng Lefty recombinant protein or treated with 200 μM SU5402 at stage 9, and analysed by WISH at stage 12. Vegetal views, dorsal to the top. (B) Four-cell embryos were injected in the two dorsal cells with 15 ng/cell of the indicated MOs, alone or in combination (7.5 ng each), together with 2.5 ng/cell FLDx. For the purposes of rescue, 5 ng of Nodal recombinant protein was injected into the blastocoele at stage 9. Injected embryos were fixed at stage 10.25 and bisected prior to WISH. Dorsal to the right (injected side), animal to the top. Movement-effector gene expression was severely downregulated in Xnr1/Xnr2 double-morphant embryos, but was rescued by the reintroduction of Nodal protein. (C) Animal caps were prepared at stage 8.5, preincubated in the presence of CHX for 1 hour, exposed to 5 ng/ml Activin A and CHX for another 2 hours, and then harvested and processed for real-time RT-PCR. Nodal activated all genes in a CHX-independent manner, except rnd1, a known indirect target of Activin signalling. Image published in: Luxardi G et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rnd1 rho6, XRnd1 Xenopus Throughout NF stage 12 endomesoderm has2 XHas2 Xenopus Throughout NF stage 12 endomesoderm pdgfra pdgfr-alpha, pdgfr2, pdgfralpha Xenopus Throughout NF stage 12 endomesoderm pcdh8 papc, paraxial protocadherin, xpapc Xenopus Throughout NF stage 12 endomesoderm Image source: Published Larger Image Printer Friendly View

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