XB-IMG-41547
Xenbase Image ID: 41547
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Fig. 3. Wnt signaling cannot caudalize in the absence of Meis3 protein activity. (A) In situ hybridization of mid-late neurula embryos injected separately at the one-cell stage with either Meis3 MO (20 ng; b,f,j,n), or mWnt3 DNA (70 pg; d,h,l,p), or both (c,g,k,o). Posterior neural marker expression of HoxB3, Krox20, N-Tub and FoxD3 is highly inhibited in Meis3 morphants (b, 25/25 embryos; f, 40/40 embryos; j, 36/36 embryos; n, 16/16 embryos). mWnt3 co-expression did not rescue the expression of these markers (c, 40/41 embryos; g, 71/75 embryos; k, 63/63 embryos; o, 29/29 embryos). Ectopic mWnt3a levels alone expand posterior marker expression (d, 20/20 embryos; h, 48/49 embryos; l, 33/36 embryos; p, 18/18 embryos). (B) sqRT-PCR of pools of 18 mid-late gastrula AC explants dissected from embryos injected at the one-cell stage with mWnt3 DNA (200 pg) and BMP DNR mRNA (100 pg), and/or separately with Meis3 MO (33 ng) (n=5 experiments). (C) sqRT-PCR of pools of 18 mid-late neurula AC explants from siblings of embryos in B (n=5 experiments). Image published in: Elkouby YM et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |