Xenbase Image ID: 41643

Figure 3. Expression Patterns of the Nodal Inhibitor Coco Vary with Leftward Flow(Aa–Ac) Coco is coexpressed with Xnr1 in lateral somitic GRP cells (cf. Xnr1 in Figures 1Ab and 1Ac).(Aa) Dorsal explant of a whole-mount in situ hybridized embryo at stage 17 with a Coco-specific antisense probe.(Ab and Ac) Histological analysis of expression domain in somitic GRP cells.(B) Representative examples of Coco mRNA expression patterns in the GRP of stage 19 embryos. Following in situ hybridization, dorsal explants were prepared and classified into right bias (R > L), equal distribution (R = L), or left bias (R < L) of Coco signals at the GRP.(C) Increase of right bias Coco expression pattern in postflow stages (left bars) is dependent on flow (right bars).(Da and Db) Wild-type leftward flow (3.4 ± 1.8 μm/s; p = 0.78) in morphants injected uni- or bilaterally with Coco-MO (cf. Movie S1), as depicted in GTT blot of representative explant (Da) and summary wind rose display of 9 embryos representing 2677 particles (Db).The following abbreviations are used: bp, blastopore; d, dorsal; e, endoderm; hyGRP, prospective hypochordal cells; l, left; LECs, lateral endodermal crest cells; no, notochord; r, right; s, presomitic mesoderm; sGRP, prospective somitic cells; v, ventral. The box in (Ab) indicates the area of higher magnification in (Ac). Color gradient in (Da) represents 25 s. Scale bar represents 50 μm. Image published in: Schweickert A et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue dand5 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco Xenopus Sometime during NF stage 17 to NF stage 19 somite gastrocoel roof plate Image source: Published Larger Image Printer Friendly View

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