XB-IMG-41859
Xenbase Image ID: 41859
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Figure 5. ESR1 Is a Target of BCL6 during LR Patterning and Neural Development(A) BCL6 MO was injected into the left side of embryos, and left LPM tissues were dissected from 20 embryos at stage 25 for quantitative RT-PCR. The injection side was traced by the coinjection of GFP (data not shown). *p < 0.05, n = 3.(B) BCL6 MO was injected into a dorsal blastomere of four-cell-stage embryos, and embryos were fixed at stage 14. Increased ESR1 expression and decreased N-tubulin expressions are indicated by white and black arrows, respectively.(C) BCL6 MO or/and Notch1 MO was injected into the left side of embryos, and left LPM tissues were dissected from 20 embryos at stage 25 for quantitative RT-PCR. *p < 0.01, n = 3; **p < 0.01, n = 3; ***p < 0.01, n = 3.(D) 1 ng ESR1-GR or Hairy2-GR was injected for each experiment.(E) 40 ng ESR1 MO or/and BCL6 MO was injected for each experiment. The injected side is indicated by “L” (left) beside the names of the injected samples. L, left; R, right; a, anterior; p, posterior.All error bars shown are standard deviation (SD) from the mean of triplicates. Image published in: Sakano D et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |