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Fig. 2. A) Superposition of en2, pax2 and XTcf-4 expression at different stages in schematic embryos. The superposition is based on the in situ hybridization depicted. B) Expression patterns of XTcf-4, pax2 and en2 as revealed by whole-mount in situ hybridization. C) Double staining of en2 (red) and XTcf-4 (blue) revealed that the expression fields of these transcription factors do not overlap. The bar indicates the cutting plane for the sections shown in D).