Xenbase Image ID: 42016

Fig. 6. miR-200b MO phenotype. A. The injection of miR-200b MO led to abnormal eye structures or even absent eyes on the injected side (white arrows). Histological sections confirmed this observation. A quantitative representation depicts the dose-dependency of this MO effect. B. The expression of Rx1, Pax6 and Sox3 was not affected upon injection of 40 ng miR-200b MO. C. At stage 23, Rx1 and Pax6 expression are downregulated after loss of miR-200b (black arrows). Other examined brain markers were not affected. D. Alcian blue staining at stage 46. MiR-200b deficient embryos showed smaller cranial cartilage structures on the injected side (black arrows). A quantitative representation is given. E. Slug and FoxD3 expression at stage 17 was not affected upon miR-200b depletion. F. The injection of 40 ng miR-200b MO had no effect on the expression of FoxD3 at stage 20 and Krox20 and Twist at stage 23. A quantitative representation is shown. n = number of independent experiments; N = number of analyzed embryos. Image published in: Gessert S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 13 optic field rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 23 eye primordium pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 13 optic field neural tube pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 23 eye primordium neural tube sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 pre-chordal neural plate chordal neural plate snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 17 neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 17 neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 23 neural crest posterior neural tube twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 23 mandibular crest hyoid crest anterior branchial crest posterior branchial crest egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 rhombomere R3 rhombomere R5 emx1l.S Xemx1 X. laevis Throughout NF stage 23 telencephalon en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 23 midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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