Xenbase Image ID: 42017

Fig. 7. miR-96 MO phenotype. A. Loss of miR-96 function was accompanied by eye malformations (white arrows). B. Injection of 20 ng miR-96 MO resulted in a slight downregulation of Rx1 and Pax6 on the injected side at stage 13 (black arrows). C. At stage 23, depletion of miR-96 led to a strong downregulation of Rx1 and Pax6 on the injected side (black arrows). Emx1 and Krox20 were not affected, whereas the expression domain of En2 was broadened in around 27% of the analyzed embryos (black arrow). D. The downregulation of miR-96 was followed by a reduction of the cranial cartilage on the MO-injected side of the embryo (black arrows). The cartilage preparation of the control MO injected embryo is identical to Fig. 6D. E. At stage 17, the expression of Slug and FoxD3 was disturbed after loss of miR-96 function (black arrows). E. At stage 20, FoxD3 expression was downregulated on the injected side. At stage 23, Krox20 and Twist were reduced after the injection of 20 ng miR-96 MO (black arrows). For all shown experiments, quantitative representations are given. n = number of independent experiments; N = number of analyzed embryos. Image published in: Gessert S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 13 pre-chordal neural plate rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 23 eye primordium pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 13 optic field neural tube pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 23 optic field neural tube sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 pre-chordal neural plate chordal neural plate snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 20 neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 20 neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 20 neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 23 migratory neural crest cell egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 rhombomere R3 rhombomere R5 twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 23 cardiac neural crest hyoid crest mandibular crest posterior branchial crest anterior branchial crest emx1l.S Xemx1 X. laevis Throughout NF stage 23 telencephalon en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 23 midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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