Xenbase Image ID: 42053

Fig. 2. Shroom3 is essential for cell shape change during gut morphogenesis. (A) Diagram of a transverse section of the stage 32 Xenopus embryo. (B) Shroom3 expression in the endoderm at stage 32. Note the robust Shroom3 expression (arrow) in the archenteron floor and roof. (C) α-tubulin staining of the archenteron floor in a control embryo. The archenteron floor displays a V-shaped morphology. (D) Archenteron floor morphology is disrupted and microtubule (MT) arrays are reduced in cells lacking Shroom3. We observed a less acute archenteron floor angle in Shroom3 MO-injected embryos (P=0.001, Mann-Whitney U-test). Moreover, archenteron floor thickness is reduced from 68±1 μm (mean ± s.e.m.; n=13) in control embryos to 59±1 μm (n=21) (P=0.0003) in Shroom3 MO-injected embryos. (E) γ-tubulin staining of the archenteron floor in a control embryo. (F) γ-tubulin accumulation is reduced in archenteron floor cells lacking Shroom3 function. (G) ZO-1 staining of tight junctions in archenteron floor cells. (H) The apical surface of archenteron floor cells is less constricted in the Shroom3 morphant embryo. ZO-1 localization is not affected. Scale bars: 10 μm. Image published in: Chung MI et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue shroom3.L apxl3, shrm, shroom, shroom-like, xshroom3 X. laevis Throughout NF stage 32 archenteron floor gastrocoel roof plate endoderm Image source: Published Larger Image Printer Friendly View

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