Xenbase Image ID: 42053
Fig. 2. Shroom3 is essential for cell shape change during gut morphogenesis. (A) Diagram of a transverse section of the stage 32 Xenopus embryo. (B) Shroom3 expression in the endoderm at stage 32. Note the robust Shroom3 expression (arrow) in the archenteron floor and roof. (C) α-tubulin staining of the archenteron floor in a control embryo. The archenteron floor displays a V-shaped morphology. (D) Archenteron floor morphology is disrupted and microtubule (MT) arrays are reduced in cells lacking Shroom3. We observed a less acute archenteron floor angle in Shroom3 MO-injected embryos (P=0.001, Mann-Whitney U-test). Moreover, archenteron floor thickness is reduced from 68±1 μm (mean ± s.e.m.; n=13) in control embryos to 59±1 μm (n=21) (P=0.0003) in Shroom3 MO-injected embryos. (E) γ-tubulin staining of the archenteron floor in a control embryo. (F) γ-tubulin accumulation is reduced in archenteron floor cells lacking Shroom3 function. (G) ZO-1 staining of tight junctions in archenteron floor cells. (H) The apical surface of archenteron floor cells is less constricted in the Shroom3 morphant embryo. ZO-1 localization is not affected. Scale bars: 10 μm.
Image published in: Chung MI et al. (2010)
Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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