Xenbase Image ID: 42055

Fig. 4. Pitx1 controls Shroom3 expression in the developing gut. (A,B) Transverse sections showing Shroom3 expression in (A) a control Xenopus embryo and (B) the Pitx1 MO-injected embryo, where Shroom3 expression is reduced in the archenteron floor (arrows). The regions indicated by the arrows in A and B approximate to those shown at higher magnification in C,E and D,F, respectively. (C) α-tubulin staining of control archenteron floor cells. (D) α-tubulin staining of archenteron floor cells lacking Pitx1. The archenteron floor fails to take on its deep V-shaped morphology, showing an average archenteron floor angle 132±4° (mean ± s.e.m.; n=22), compared with 62±5° (n=16) (P<0.0001) in control embryos. Moreover, the archenteron thickness is reduced from 90±3 μm (mean ± s.e.m.; n=13) to 68±3 μm (n=17) (P<0.001). (E) γ-tubulin staining of the archenteron floor in the control embryo. (F) γ-tubulin accumulates less in archenteron floor cells lacking Pitx1. (G) ZO-1 staining of control archenteron floor cells. (H) The apical surface of archenteron floor cells is less constricted in the Pitx1 knockdown embryo, whereas ZO-1 localization is not affected. Scale bars: 10 μm. Image published in: Chung MI et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue shroom3.L apxl3, shrm, shroom, shroom-like, xshroom3 X. laevis Throughout NF stage 32 archenteron floor gastrocoel roof plate Image source: Published Larger Image Printer Friendly View

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