Xenbase Image ID: 42240

Figure 5. Cooperation between xHtrA1 and FGF Signals(A) Ectopic tail-like outgrowth (arrowhead) induced after a single ventral injection of pCS2-FGF4 DNA (4 pg) at the 4-cell stage.(B) Expansion of Xbra expression induced by FGF4 mRNA (6 pg).(C) Ectopic N-tubulin expression induced by FGF8 mRNA (5 pg).(D–G) Animal caps extracted from stage 8 embryos and cultured until stage 25. Caps extend only slightly when injected with xHtrA1 (360 pg) or FGF4 (3.2 pg) but show robust elongation when injected with both mRNAs together.(H) RT-PCR of animal cap explants at stage 11.(I and J) Animal injection of XFD mRNA at the 4-cell stage (110 pg) causes enlargement of head structures.(K and L) XFD restores head development and blocks ectopic tail outgrowth induced by xHtrA1 mRNA (80 pg).(M and N) A single marginal injection of XFD mRNA (220 pg) disrupts Xbra expression.(O and P) XFD inhibits ectopic Xbra expression by xHtrA1 mRNA (80 pg).(Q and R) A single animal injection of DnFGFR4a mRNA (110 pg) suppresses N-tubulin expression. nlacZ mRNA was coinjected as lineage tracer (red nuclei).(S and T) DnFGFR4a blocksN-tubulin expression induced by xHtrA1 mRNA (80 pg) on the injected side.Frequency of embryos with the indicated phenotype was A, 13/66; B, 23/23; C, 118/120; J, 54/68; K, 68/80 (head reduction) and 32/80 (ectopic tail), L, 13/16; N, 38/47; O, 13/13; P, 16/18; R, 18/18; S, 10/12; and T, 10/10. Image published in: Hou S et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 marginal zone mesoderm Image source: Published Larger Image Printer Friendly View

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