Xenbase Image ID: 42246

Fig. 3. Overexpression of XtSulf2 affects the expression of a number of genes expressed in anterior neural tissue and the cranial neural crest. One blastomere of a two-cell Xenopus tropicalis embryo was injected with 0.5 ng of XtSulf2 mRNA, embryos were allowed to develop to NF stage 28 and fixed for in situ hybridization using En2 (A–B), Isl1 (C–D), Krox20 (E–G), Lim1 (H–I), NeuroD (J–L), Otx2 (M–N), Pax6 (O–Q), Slug (R–S), Sox3 (T–U), and Sox8 (V–W). En2 expression is shifted posteriorly on the injected side (A–B). Isl1 expression in the profundal and trigeminal placodes shifts posteriorly (black arrowheads) as does the expression in the heart (C–D). Krox20 expression in the migrating neural crest shifts posteriorly (E–F white arrowheads) as does the expression in r5 (G black lines). Lim1 expression in the pronephros is restricted anteriorly (H–I black lines). NeuroD expression in the profundal and trigeminal placodes is disrupted (J–K red arrowheads), and expression in the olfactory placodes fuses with expression in the eye placodes (L yellow arrowheads). Otx2 expression is restricted in the midbrain (M–N black lines). Pax6 expression is ectopically expressed in the posterior and dorsal head ectoderm (O–P–Q red asterisk). Slug expression at the leading edge of neural crest migration remains closer to the neural tube than in controls (R–S black lines). Sox3 expression is decreased in neural crest but expands laterally (T–U black lines). Sox8 expression in the anterior and posterior branchial arches is decreased and the leading edge of the migrating neural crest remains closer to the neural tube than in controls (V–W green arrowhead). (A–B, G, Q) are dorsal views of embryos, anterior at the top. (C–F, H–K, M–P, R–W) are lateral views of embryos, anterior to the left. (L) is an anterior view of embryo in (J–K), dorsal at the top. Asterisks indicate injected side. CS control side, IS injected side. Image published in: Guiral EC et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue isl1 islet-1, islet1, Xisl-1, Xislet-1 X. tropicalis Throughout NF stage 28 trigeminal placode cranial placode pineal gland profundus ganglion heart egr2 EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. tropicalis Throughout NF stage 28 hindbrain anterior neural tube neuroectoderm cranial neural crest rhombomere R5 rhombomere R3 migratory neural crest cell neurod1 neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. tropicalis Throughout NF stage 28 trigeminal placode midbrain pineal gland nervous system eye neuroectoderm lens placode profundus ganglion olfactory placode epibranchial placode otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. tropicalis Throughout NF stage 28 midbrain eye lens placode brain forebrain sox3 Sry, Xlsox3, xSox3, Xtsox3 X. tropicalis Throughout NF stage 28 neural tube neuroectoderm lens placode epibranchial placode lateral line placode cranial neural crest eye spinal cord brain forebrain midbrain hindbrain lens anterior branchial crest posterior branchial crest sox8 X. tropicalis Throughout NF stage 28 neural tube neuroectoderm cranial neural crest mandibular crest hyoid crest trunk neural crest eye lateral line placode anterior branchial crest posterior branchial crest trigeminal placode epibranchial placode forebrain en2 En-2, en2-a, en2-b, eng2, engrailed 2, engrailed-2, engrailed homolog 2, maben, xen2 X. tropicalis Throughout NF stage 28 anterior neural tube neuroectoderm midbrain-hindbrain boundary brain lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 X. tropicalis Throughout NF stage 28 pronephric kidney pronephric duct neural tube tail bud hindbrain anterior neural tube pax6 an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. tropicalis Throughout NF stage 28 neuroectoderm neural tube anterior neural tube posterior neural tube brain forebrain midbrain hindbrain central nervous system spinal cord eye snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 28 cranial neural crest neuroectoderm neural tube posterior neural tube anterior neural tube hindbrain Image source: Published Larger Image Printer Friendly View

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