Xenbase Image ID: 42247

Fig. 4. XtSulf2 overexpression disrupts cranial neural crest migration. One blastomere of a two-cell X. tropicalis embryo was injected with 0.5 ng of XtSulf2 mRNA, embryos were allowed to develop to NF stage 22 and 30 and fixed for in situ hybridisation using HoxB3 (A–E), NeuroD (F–I), Slug (J–M), Sox8 (N–Q) and Twist (R–U). (A–B, F–G, J–K, N–O, R–S) Lateral views of control and injected sides of NF stage 22 embryos. (E) Dorsal view of embryo in (A–B), black asterisk indicated the injected side. (C–D, H–I, L–M, P–Q, T–U) Lateral views of control and injected sides of NF stage 30 embryos. At stage 22, on the control side 6–12% of embryos show disrupted migration, while on the injected side 58–71% of migration is found to be inhibited (n = 55–84) as assayed by the expression of CNC markers shown. At stage 30, on the control side 4–5% of embryos show disrupted migration, while on the injected side 40–53% of migration is found to be inhibited (n = 34–81). Image published in: Guiral EC et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 22 cranial neural crest mandibular crest hyoid crest branchial crest neuroectoderm neural tube posterior neural tube anterior neural tube trigeminal ganglion cranial placode sox8 X. tropicalis Throughout NF stage 22 cranial neural crest neural tube neuroectoderm mandibular crest hyoid crest branchial crest anterior neural tube tail bud trunk neural crest pronephric mesenchyme foregut endoderm twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Throughout NF stage 22 cranial neural crest neuroectoderm mandibular crest hyoid crest branchial crest hoxb3 hox2.7, hoxb-3, LOC108700804, LOC108702517 X. tropicalis Throughout NF stage 22 hindbrain neural tube rhombomere R5 cranial neural crest neuroectoderm neurod1 neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. tropicalis Throughout NF stage 29 and 30 eye primordium pineal gland neuroectoderm lens placode trigeminal placode profundus ganglion lateral line placode olfactory placode hoxb3 hox2.7, hoxb-3, LOC108700804, LOC108702517 X. tropicalis Throughout NF stage 29 and 30 neural crest cranial neural crest neural tube hindbrain neuroectoderm rhombomere R5 neurod1 neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. tropicalis Throughout NF stage 22 neuroectoderm eye primordium trigeminal placode profundal placode lateral line placode lens placode neural tube snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 29 and 30 cranial neural crest neural tube mandibular crest hyoid crest anterior branchial crest posterior branchial crest periocular region chordoneural hinge sox8 X. tropicalis Throughout NF stage 29 and 30 neural crest cranial neural crest neural tube brain forebrain midbrain midbrain-hindbrain boundary trunk neural crest ventral endoderm foregut pronephric mesenchyme chordoneural hinge dorsal pancreatic bud ventral pancreatic bud twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Throughout NF stage 29 and 30 cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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