Xenbase Image ID: 42250

Fig. 7. Delay in cranial neural crest migration in XtSulf1/2 double knockdown embryos is rescued by XtSulf2 mRNA injections. The CNC migrates into the mandibular, hyoid and anterior and posterior branchial arches in control and CMO embryos (A–D). In Sulf 1/2 double knockdown embryos, the CNC migration is blocked (E–F), migration is rescued with XtSulf2 mRNA (G–H). Xenopus tropicalis embryos were injected at the two- and four-cell stage into the animal hemisphere with either 30 ng of CMO (C, D), 10 ng of S1 AMO and 20 ng of S2 AMO (E, F) or 10 ng of S1 AMO, 20 ng of S2 AMO and 1.5 ng of XtSulf-2 mRNA (G, H). Neural crest migration was assayed by analysing Twist expression by in situ hybridisation. All panels show lateral views with anterior to the left. A summary of the results shown in this figure are reported in Table 2. Image published in: Guiral EC et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Sometime during NF stage 22 to NF stage 29 and 30 cranial neural crest mandibular crest hyoid crest branchial crest anterior branchial crest posterior branchial crest migratory neural crest cell Image source: Published Larger Image Printer Friendly View

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