Xenbase Image ID: 42356

Fig. 2. Analysis of XADAMTS1 expression. (A) Northern blot analysis. Total RNA (10 μg) from different developmental stages was loaded in each lane. Bands of 18S rRNA stained with ethidium bromide were used for a loading control. (B) Whole-mount in situ hybridization for XADAMTS1 mRNA during blastula to neurula stages. (a, b) Late blastulae (stage 8.5) bisected vertically after staining for XADAMTS1 (a) and FGF8 (b). Magnified images of the enclosed regions are shown in the inset. (c) Comparison of expression domains between XADAMTS1 and Xlim-1 at the gastrula stage (stage 10.5). Embryos were bisected into left and right halves and subjected to whole-mount in situ hybridization for XADAMTS1 (left panel) and Xlim-1 (right panel), respectively. Dorsal is to the right (XADAMTS1) or the left (Xlim-1). (d) Vegetal view of a mid-gastrula embryo (stage 11.5) cleared with BB/BA. (e–g) Late gastrulae (stage 12) bisected sagittally after staining for XADAMTS1 (e), FGF4 (f), and FGF8 (g). Dorsal is upwards; anterior is to the left. (h) Dorsal view of neurula (stage 18). Anterior is upwards. (C) Expression patterns of XADAMTS1 at tailbud stages. (a) Early tailbud stage embryo (stage 26). Transverse (b–d) and horizontal (e) sections of stained tailbud stage embryos (stage 26). (f) Late tailbud stage embryo (stage 31) cleared with BB/BA. (g) Transverse section of a stained late tailbud stage embryo (stage 31). Dorsal is upwards (a–d, f, g). Anterior is to the left (a, e, f). ba, branchial arch; d, dermatome; e, eye; h, heart anlage; n, notochord; nt, neural tube; ov, otic vesicle; pn, pronephros. Arrowheads indicate the dorsal blastopore. Image published in: Suga A et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue adamts1.L xadamts1 X. laevis Sometime during NF stage 8 to NF stage 11 sensorial layer of neurectoderm neuroectoderm outer layer ectoderm dorsal mesoderm ventral lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 10.5 mesoderm dorsal ventral sensorial layer of neurectoderm involuted dorsal mesoderm blastopore lip upper blastopore lip adamts1.L xadamts1 X. laevis Throughout NF stage 12 ectoderm mesoderm marginal zone dorso-lateral marginal zone neuroectoderm fgf4.L efgf, fgf-4, fgf4-a, fgf4-b, fgfe, HBGF-4, XEFGF, Xfgf-4, Xfgf4 X. laevis Throughout NF stage 12 mesoderm fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 12 upper blastopore lip involuting marginal zone mesoderm lower blastopore lip adamts1.L xadamts1 X. laevis Throughout NF stage 26 brain dermatome hindbrain cranial neural crest tail region fin branchial crest hyoid crest adamts1.L xadamts1 X. laevis Throughout NF stage 31 heart primordium pronephric kidney neural tube posterior neural tube anterior neural tube tail fin dorsal tail fin optic vesicle dermatome brain hindbrain branchial arch head region olfactory placode adamts1.L xadamts1 X. laevis Throughout NF stage 18 embryo posterior Image source: Published Larger Image Printer Friendly View

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