Xenbase Image ID: 42382

Fig. 1. Expression patterns of xMID1 and xMID2 through early embryogenesis. (A,B) RT-PCR analysis. (A) xMID1 and xMID2 mRNAs are expressed maternally and zygotically. Ornithine decarboxylase (ODC) was used as an internal control. The number above each lane is the embryonic stage. â RT, control experiment without reverse transcriptase. (B) Ectodermal expression of xMIDs in the early neurula (stage 14) Xenopus embryo. In the neural plate, xMID1 and xMID2 were expressed at similar levels to in ventral epidermis (Epidermis) and whole embryo (W.E.). NCAM and Sox2 are pan-neural markers. Epidermal keratin I (Epi. keratin) is an epidermal marker. (C-I) Expression pattern of xMID1 in embryos at the stages indicated (lower right in each panel). (C) Dorso-posterior view, dorsal to the top. (D,E) Dorsal view, anterior to the top. (F) Anterior view, dorsal to the top. (G) Dorsal view, labeled with sense probe (S), anterior to the top. (H,I) Lateral view, anterior to the left, dorsal to the top. (J,K) xMID2 expression. Lateral view, anterior to the left, dorsal to the top. (K) Sense probe (S). cg, cement gland; hb, hindbrain; ht, heart tube; mb, midbrain; nt, neural tube; op, optic vesicle; ot, otic vesicle; pg, pineal gland; pn, pronephros; pp, pharyngeal pouch; pr, proctodeum; so, somite. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue mid1.L bbbg1, fxy, gbbb1, midin, midline 1, ogs1, osx, rnf59, trim18, xprf, znfxy X. laevis Throughout NF stage 13 ectoderm epidermis blastopore mid1.L bbbg1, fxy, gbbb1, midin, midline 1, ogs1, osx, rnf59, trim18, xprf, znfxy X. laevis Throughout NF stage 15 presumptive paraxial mesoderm neural plate neuroectoderm blastopore mid1.L bbbg1, fxy, gbbb1, midin, midline 1, ogs1, osx, rnf59, trim18, xprf, znfxy X. laevis Throughout NF stage 20 paraxial mesoderm somite trunk somite neural tube anterior neural tube otic region optic field cement gland primordium mid1.L bbbg1, fxy, gbbb1, midin, midline 1, ogs1, osx, rnf59, trim18, xprf, znfxy X. laevis Throughout NF stage 25 brain midbrain otic placode eye ventral retina cement gland primordium tail somite presomitic mesoderm tail bud epidermis mid1.L bbbg1, fxy, gbbb1, midin, midline 1, ogs1, osx, rnf59, trim18, xprf, znfxy X. laevis Throughout NF stage 29 and 30 heart primordium optic vesicle brain hindbrain midbrain-hindbrain boundary cement gland tail somite tail tip pronephric kidney pronephric duct eye ventral retina presomitic mesoderm spinal cord epidermis mid2.L fxy2, midline 2, rnf60, trim1 X. laevis Throughout NF stage 32 brain midbrain hindbrain otic vesicle heart head region pineal gland Image source: Published Larger Image Printer Friendly View

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