Xenbase Image ID: 42385

Fig. 4. xMIDs regulate the localization of adhesive molecules. (A-H′) Transverse sections through the neural plate of unilaterally control-Mo-injected (Cont.-Mo) (A,C,E,G) and xMID-Mo-injected (B,D,F,H) Xenopus embryos at stage 15.5, stained with antibodies against ZO-1 (A,B), C-cadherin (C-cad.) (C,D), β-catenin (β-cat.) (E,F), or laminin (G,H). Flag-β-globin mRNA (250 pg) was co-injected as a tracer, and stained with an anti-Flag antibody (magenta). (A-F) Dashed lines indicate Flag-positive Mo-injected cells. (H) Arrowheads indicate the attenuation of laminin accumulation basal to the xMID-Mo-injected cells. (H′) Higher-magnification view of the boxed area in H. Scale bars: 50 μm. (I-L) Quantification of marker intensities in the morphants at stage 15.5. For ZO-1 intensity: control-Mo, n=27 sites (3 embryos); xMID-Mo, n=44 sites (6 embryos). For C-cadherin: control-Mo, n=16 sites (4 embryos); xMID-Mo, n=20 sites (5 embryos). For β-catenin: control-Mo, n=7 sites (3 embryos); xMID-Mo, n=15 sites (4 embryos). For laminin: control-Mo, n=19 sites (4 embryos); xMID-Mo, n=19 sites (3 embryos). *P<0.05, **P<0.001. (M,N) Schematic illustrations showing the control (M) and xMID (N) morphants. Rectangles indicate the regions analyzed in this study. so, somite; nt, notochord. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue cdh3.S b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, P-cadherin, pcad, U-cadherin, xb-cad, XB-cadherin, XBcad X. laevis Sometime during NF stage 15 to NF stage 16 neural crest neuroectoderm tjp1.S zo-1, zo1 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neural groove neuroectoderm ctnnb1.S B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neuroectoderm lamb2.L lams X. laevis Sometime during NF stage 15 to NF stage 16 neural plate neuroectoderm Image source: Published Larger Image Printer Friendly View

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