XB-IMG-42386
Xenbase Image ID: 42386
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Fig. 5. xMIDs associate with and regulate microtubules. (A) Transverse section through the neural plate of a Xenopus embryo injected with EGFP-xMID1 mRNA (50 pg) at stage 15.5, and stained with anti-GFP and anti-α-tubulin antibodies. Scale bar: 20 μm. (B-E) Transverse sections through the neural plate of embryos unilaterally injected with control-Mo (B,C) or xMID-Mo (D,E), and stained for α-tubulin (B,D) or acetylated tubulin (Ac.-Tubulin) (C,E) antibodies. Flag-β-globin mRNA (250 pg) was co-injected as a tracer, and stained with an anti-Flag antibody (middle panels). Bottom panels show traced drawings of cells stained by α-tubulin and anti-acetylated tubulin antibodies. (F) Western blots of immunoprecipitates (IP) or lysates from embryos expressing EGFP-tubulin (EGFP-tub.) mRNA (1 ng), detected with anti-GFP and anti-acetylated tubulin (Acetylated-tub.) antibodies. Immunoprecipitation was performed using the anti-GFP antibody. (G) Quantification of immunoprecipitation assay, showing the signal intensities of acetylated tubulin from three independent experiments, normalized to those of EGFP-tubulin. Error bars indicate s.e.m. *P<0.01. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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