XB-IMG-42387
Xenbase Image ID: 42387
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Fig. 6. Mig12 functions with xMIDs in neural tube closure. (A,B) Dorsal views of Xenopus embryos given bilateral injections of control-Mo (A) or Mig12-Mo (B); anterior is to the top. (C) Average distance between the neural folds of unilaterally injected Mig12 morphants was reduced by the Mo-insensitive Mig12 mRNA (25, 50 pg). (D-H) Synergistic effects of Mig12-Mo and xMID-Mo on neural tube closure. Dorsal views, anterior to the top. Injection of low doses (8.4 ng) of Mig12-Mo (E) or xMID-Mo (F) caused slight delays in neural tube closure. Co-injection of Mig12-Mo and xMID-Mo (8.4 ng each) induced severe NTDs (G,H). (I) Average distance between the neural folds of unilaterally injected Mig-12 morphants was reduced dose-dependently by xMID1 mRNA (50, 100, 250, 500, 1000 pg). (J) Average distance between the neural folds of unilaterally injected xMID morphants was not reduced by Mig12 mRNA (25, 50, 100, 250, 500 pg). The number of embryos examined in C,H,I,J is indicated above each bar. (K-M) Transverse sections through the neural plate of embryos given unilateral injections of control-Mo (K, 8.4 ng), Mig12-Mo (L, 17 ng), or Mig12-Mo and xMID-Mo (M, 8.4 ng each), stained with α-tubulin (top) and anti-GFP (middle) antibodies. mRNA (125 pg) encoding membrane-bound EGFP was co-injected as a tracer. Bottom panels show traced drawings of cells stained by anti-α-tubulin. Image published in: Suzuki M et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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