Xenbase Image ID: 42394

Fig. 6. Phenotype of XCR1 and XCR3 morphants. Embryos were injected at the 1-cell stage with a total of 40 ng of the indicated morpholinos and mRNAs together with GFP mRNA as a tracer. (A) Depletion of XCR1 or XCR3 or both impairs gastrulation. GFP positive embryos were sampled when MO C-injected embryos reached stage 11. (B) Expression of XCR1 and XCR3 rescues the phenotype of embryos depleted for XCR1 and XCR3. (C) Only XCR1 morphants develop further than gastrulation. Embryos were injected as in panel A and scored for survival. (D) Phenotype of XCR1 morphants at neurula stages (stage 16, upper panels) and tailbud stages (stage 45, lower panels). (E and F) Whole-mount in situ hybridisation analysis of the expression of mesendodermal markers (XFKH1, Xnot, Xbra and Mixer) or the neuronal marker (N-tubulin) at stage 10.5 (E) and stage 16 (F). Pictures show a vegetal view of embryos at stage 10.5 and 11; for stage 16 embryos, dorsal view with anterior up and for tailbud, lateral view. Image published in: Dorey K and Hill CS (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxa4 fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 Xenopus Throughout NF stage 10.5 endomesoderm not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot Xenopus Throughout NF stage 10.5 endomesoderm tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 10.5 endomesoderm mixer tMixer Xenopus Throughout NF stage 10.5 endomesoderm not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot Xenopus Throughout NF stage 16 mesoderm Image source: Published Larger Image Printer Friendly View

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