Xenbase Image ID: 42750

Fig. 4. Deep versus superficial hybridization. Stage 14 embryos were fixed and hybridized in whole mount in situ to probes for (A) TFAP2α and (C) TFAP2γ. Conditions were adjusted to optimize deep cell hybridization: Proteinase K treatment was increased slightly, and the color development time was increased. For Slug (D) and TFAP2β (B), the images were magnified from Fig. 3B. Following hybridization embryos were embedded in JB4 and cut into 10 μ sections. The sections shown were located 300 μ from the anterior tip of the embryos. Hybridization to superficial cells is indicated with black arrows, and to deep, NC cells with red. The bar in (D) represents 100 μ. Image published in: Zhang Y et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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