XB-IMG-43256
Xenbase Image ID: 43256
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Fig. 5. Cyclopamine treatment expands hypaxial specific markers medially and posteriorly. The expressions of lbx1 (A–Q), tbx3 (R, S), and sim1 (T, U) were examined in control (A, B, E, F, I, J, M, N, R, T) and cyclopamine-treated (C, D, G, H, K, L, O, P, Q, S, U) embryos. Trunk somites in lbx1- and tbx3-probed embryos are labeled with numbers, with the most anterior trunk somite labeled 1. At stage 26 there are no significant differences in lbx1 expression between control (A, B) and cyclopamine-treated embryos (C, D). At stages 31 (E–H), 33/34 (I–L), and 37/38 (M–Q), lbx1 expression is expanded one somite posterior compared to controls, and is expanded medially towards the notochord (P, arrow, compare to N). The image in panel Q is a higher power image of the embryo in panel O. The expression of tbx3 is also expanded one somite posteriorly in cyclopamine-treated embryos (S compared to R). The expansion of the hypaxial dermomyotome marker sim1 is also observed in stage 32 cyclopamine-treated embryos (U) in both the anterior somites where it is normally expressed (arrowhead) and in the posterior somites (arrow), as compared to a control embryo (T). Image published in: Martin BL et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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