XB-IMG-43349
Xenbase Image ID: 43349
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Fig. 5. Effects of Cdx knockdown on Hox gene expression. Cdx function is required for the normal expression of multiple posterior Hox genes. A–E: Whole-mount in situ hybridizations of the indicated Hox genes. A: Late gastrula stage 13 embryos, and B–E show late neurula stage 20 embryos. A–C: Embryos were injected bilaterally with 20 ng of Cdx1, Cdx2, and Cdx4 MOs. A: Panels with HoxA9 shows posterior views with dorsal to the top. Panels with HoxB9 show dorsal views with anterior to the top. Black bars indicate lateral extent of expression domain in neural tube. B: Lateral views with anterior to the left. C: Dorsal views, anterior to the left. D: Representative control embryos and embryos injected unilaterally with 10 ng of Cdx1, Cdx2, Cdx4 MOs, or the triple Cdx MO combination; injected side is indicated with a red asterisk (left panels are dorsal views with anterior to the left, and right panels are posterior views with anterior to the top. E: A control uninjected embryo and embryos injected bilaterally with 20 ng of Cdx1, Cdx2, or the Cdx1 and Cdx2 combination (dorsal views with anterior to the left). Black arrows indicate anterior boundary of expression within the neural tube on injected and contralateral uninjected sides. Image published in: Faas L and Isaacs HV (2009) Copyright © 2009 Wiley-Liss, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |