XB-IMG-43350
Xenbase Image ID: 43350
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Fig. 6. Effect of Cdx knockdown during gastrulation. A: The major morphogentic movements of gastrulation are not affected in Cdx knockdown embryos. Sagittal sections are shown of a control embryo and an embryo injected with a total dose of 40 ng of the triple Cdx1+2+4 MO combination at late gastrula stage 13 (anterior to the left, dorsal to the top). ar, archaenteron; ntc, prospective notochord; blc, blastocoel; bp, blastopore; end, endoderm. B: Growth factors induce elongation in animal cap explants from Cdx knockdown embryos. Animal hemisphere explants were taken at mid-blastula stage 8 from uninjected embryos and embryos injected with 40 ng of Cdx1, Cdx2, and Cdx4 MOs and cultured to neurula stage 18. Growth factor treatment was with 10 U/ml recombinant FGF4 or 50 U/ml recombinant activin-A (act). C: The expression of early germ layer markers is unaffected in Cdx knockdown embryos. Expression is shown of the endodermal marker Xsox17b and the mesodermal markers Xhox3 and brachyury (Xbra) in triple Cdx knockdown embryos (40-ng total dose) and controls (posterior views, dorsal to the top). Xbra, Xhox3, and Xsox17b embryos are at gastrula stages 11.5, 12.5, and 11, respectively. Image published in: Faas L and Isaacs HV (2009) Copyright © 2009 Wiley-Liss, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |