XB-IMG-43412
Xenbase Image ID: 43412
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Fig. 2. Temporal and spatial expression of Xhip. Whole mount in situ hybridization of Xhip in Xenopus embryos. Staging according to Nieuwkoop and Faber (1967). (a) Neurula stage (NF stage 18), anterior view. White arrowhead, preplacodal ectoderm; black arrowhead, expression of Xhip in paraxial stripes in the ventral neural tube. (b) Early tailbud (NF stage 20), frontal view. Arrowhead, presumptive placodal epithelium. (c) Dorsal view, anterior to the left. Arrow, lateral mesoderm; arrowhead, ventral neural tube. (c′) Transversal section. White arrowhead, chorda dorsalis; arrow, lateral mesoderm; black arrowheads, paraxial stripes in the ventral neural tube. (d) Mid-tailbud (NF stage 24), frontal view. Arrow, diencephalon. (e) Lateral view, anterior to the left. White arrowhead, pAD + pM, common anterodorsal and middle lateral line placode; white arrowhead, pP, posterior lateral line placode. (c′, e′, f) Transversal sections reveal Xhip expression in the chorda dorsalis at NF stages 18 and 24, but not at NF stage 28 (white arrowheads). (f) Expression of Xhip in the roof plate (white arrow), Xhip dorsal to the floor plate (black arrowhead). (g) In tailtip stage embryos (NF stage 34), frontal view, Xhip is expressed in the pineal gland (white arrow), in the anterodorsal lateral line placode (white arrowhead), in the olfactory placodes (black arrowhead) and in the anterior head mesenchyme (black arrow). (h) Lateral view, anterior to the left. A black arrowhead indicates Xhip expression in the olfactory placode. White arrow, pineal gland anlage. White arrowheads, anterodorsal, middle and posterior lateral line placode; black arrows from anterior to posterior, facial epibranchial placode fused with the anteroventral lateral line placode, glossopharyngeal placode, 1st, 2nd, and 3rd vagal epibranchial placode. (i) Ventral view of the head of the tadpole shown in j (NF stage 41); arrowhead, lower jaw; arrow, rim of the mouth opening. (j) Lateral view, anterior to the left. Black arrowhead, lower jaw; white arrowhead, rim-like staining for Xhip at the transition zone between the yolk mass and the ectoderm. (k) Ventral view of the proctodeum of the tadpole shown in j, white arrowhead as in j. Abbreviations: 1,2,3ep, 1st, 2nd, and 3rd vagal epibranchial placode; ahm, anterior head mesenchyme; cd, chorda dorsalis; de, diencephalon; fep, facial epibranchial placode fused with the anteroventral lateral line placode; gep, glossopharyngeal placode; mop, mouth opening; lj, lower jaw; lme, lateral mesoderm; pAD, anterodorsal lateral line placode; pgl, pineal gland; pM, middle lateral line placode; pOl, olfactory placode; pP, posterior lateral line placode; ppe, preplacodal ectoderm; ppl, presumptive placodal epithelium; pr, proctodeum; rp, roof plate; vnt, ventral neural tube. Image published in: Cornesse Y et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |