XB-IMG-43989
Xenbase Image ID: 43989
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Figure 4. Early marginal zone markers and mesodermal tissue differentiation are not affected by Xdd1. (a) Whole-mount immunochemical staining with muscle-specific 12/101 antibodies. Top: control embryo; bottom: two embryos injected dorsally with Xdd1 mRNA. Arrowhead points to a somite. (b) Cross-section of an Xdd1-injected embryo after whole-mount staining with notochord-specific MZ15 antibodies. Brown staining outlines a sheath around notochord (nc). Bar in (b), which also refers to (d), is 150 μm. (c) Whole-mount staining with MZ15 antibodies. Top left: four embryos injected with Xdd1 mRNA; white arrowhead points to notochord. Bottom left: two embryos injected with wild-type Xdsh mRNA; control uninjected embryo is shown on the right. (d) Cross-section of a control uninjected embryo stained with MZ15 antibodies. (e) Northern analysis of early marginal zone markers in control embryos (lane 1) and in embryos injected dorsally with Xdd1 (lane 2) or Xdsh (lane 3) mRNAs. Total RNA was prepared when control embryos reached stage 10.5. Xbra is a general marginal zone marker. Xotx2, goosecoid (Gsc), Xlim 1 and chordin are dorsal marginal zone markers. Xnr3 is expressed in the superficial layer of the Spemann organizer. Fibronectin (FN) mRNA is a control for loading. (f) Xdd1 does not alter the localization of chordin transcripts. In situ hybridization of stage 10.5 gastrula embryos with chordin anti-sense RNA probe. Far left: uninjected albino embryo; centre left: uninjected pigmented embryo; right: two pigmented embryos injected with Xdd1 mRNA in the dorsal margin at the two- to four-cell stage. Image published in: Sokol SY (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V.
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