Xenbase Image ID: 44154

Figure 7. A-E prime : X-Delta-2 loss of function affects cranial neural crest cell migration. Noninjected (Nic) controls (A-E) and embryos injected on the left-hand side with X-Delta-2 morpholino (MO; A prime -E prime ) were analyzed for the expression of cranial neural crest markers or stained with Alcian blue to highlight the cartilage. A,A prime ,B,B prime show stage 21 embryos from the anterior side with dorsal to the top; C,C prime ,D,D prime show stage 25 embryos from the lateral side with dorsal to the top; and in E,E prime show stage 48 embryos from the ventral side with anterior to the top. Cranial neural crest cells fail to migrate as shown by the expression of X-Slug (A prime ), Xsnail (B prime ), and X-dll4 (C prime ,D prime ). The cartilage staining shows that the gills, derived from the branchial arches, do not form in the X-Delta-2 MO-injected side (arrow in E prime ). The asterisk indicates the injected side. Nic for X-dll4 in C and D is the noninjected side of the same embryos shown in C prime ,D prime , respectively. Image published in: Peres JN and Durston AJ (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 21 cranial neural crest migratory neural crest cell snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 21 cranial neural crest migratory neural crest cell neural plate border pre-chordal neural plate border chordal neural plate border dlx2.L tes-1, tes1, X-dll4, Xdll4 X. laevis Sometime during NF stage 21 to NF stage 25 otic vesicle cranial neural crest migratory neural crest cell mandibular crest hyoid crest anterior branchial crest posterior branchial crest Image source: Published Larger Image Printer Friendly View

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