XB-IMG-44257
Xenbase Image ID: 44257
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FIG. 4. Mislocalization of multiple vegetal mRNAs with VegT depletion. Whole-mount in situ hybridization analysis of Bicaudal-C (A C;
BicC), Xotx1 (D F), Wnt11 (G I), and Xcat2 (J L) localization in uninjected oocytes (Control) and oocytes injected with 30 ng of morpholino
oligo (VegT Morpholino) or 7 ng of chimeric oligo (VegT Thioate). The results shown are representative of three independent experiments
(n 5 15 20 per sample in each experiment). Vegetal localization of each mRNA was observed in 100% of uninjected and morpholino
oligo-injected oocytes. While vegetal localization of Xotx1 and Xcat2 was observed in 95 100% of chimeric oligo-injected oocytes,
localization of Bicaudal-C and Wnt11 was observed in less than 10% of oocytes. Vegetal views are shown. Scale bar, 0.5 mm. Image published in: Heasman J et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |