Xenbase Image ID: 44292

Figure 3. Stage-dependent BMP inhibition in the developing pronephros does not alter expression of glomal-specific genes. Embryos were co-injected with 300-400 pg Delta Smad7tevGR, 10 pg TEV2GR, and beta -galactosidase, and cultured in the presence of DEX starting at stage 14. The effect of BMP inhibition in the pronephric glomus was assessed by in situ hybridization to evaluate expression of the glomal markers WT-1 (A-C), nephrin (D,E), and neph1 (F,G). No observable difference in glomal marker expression between the injected and uninjected control side was observed following regulated inhibition of BMP signaling (A-G). C: Because the glomus is located more medially and is thus more difficult to unequivocally evaluate in whole mount, we examined glomal marker expression in section. Following in situ hybridization, embryos were embedded in JB-4 plastic and sectioned transversely into 30- mu M sections (at the line in B). C shows a representative embryo exhibiting similar levels of WT-1 expression on both the injected and uninjected sides of the tadpole. Nt, neural tube; nc, notochord. Percent indicates the percentage of injected, DEX-treated embryos in which the reported phenotype was observed. Image published in: Bracken CM et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page