XB-IMG-44295
Xenbase Image ID: 44295
Figure 6. BMP inhibition has varying effects on somitic and lateral plate mesoderm pre- and post-gastrulation. mRNAs encoding Delta Smad7tevGR (300-400 pg), TEV2GR (10 pg), and the lineage marker beta -galactosidase (B,C,F: pink; E: light blue) were injected into a single blastomere at the 4-cell stage. DEX was added to induce Delta Smad7-mediated BMP inhibition either at onset of gastrulation (stages 9-10) or at early neurula (stage 14). At the appropriate stages, embryos were assessed for somitic protein 12/101 or heart (lateral plate derivative) transcript cardiac actin by immunohistochemistry or in situ hybridization, respectively. Panels A and D show the uninjected sides of embryos. Inhibition of BMP signaling at either stage 10 or stage 14 in the lateral plate mesoderm resulted in a loss of cardiac actin expression (C,F, black arrows). BMP inhibition in somitic mesoderm at stage 10 resulted in mispatterned somites (B, arrowheads), while similar inhibition at stage 14 had no effect on somitic mesoderm protein 12/101 (E). Percentage of embryos in each panel refers to the percentage of embryos expressing indicated phenotype. Image published in: Bracken CM et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |