Xenbase Image ID: 44337

Fig. 5. Compared to uninjected embryos (A and top embryo in part H), the unilateral injection of either Snail2 (B) or Twist (C) morpholinos (7 ng/embryo) in two-cell embryos led to the anterior loss of myoD RNA in situ hybridization staining in stage 22/24 embryos. The loss of myoD staining in Twist (D) or Snail2 (E) morphant embryos was rescued by Twist RNA injection (500 pg/embryo); similarly the loss of myoD staining in Snail2 (F) or Twist (G) morphant embryos was rescued by the injection of Snail2-GFP RNA (500 pg/embryo). All embryos are oriented with anterior to the left and dorsal to the top. (H) In larvae derived from unilaterally injected 2-cell embryos there was a pronounced curvature toward the injected side. In all cases, it is worth noting that these embryos survived gastrulation and are therefore likely to represent partial, rather than complete, loss of function phenotypes. Image published in: Zhang C and Klymkowsky MW (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Sometime during NF stage 22 to NF stage 24 somite Image source: Published Larger Image Printer Friendly View

Return to previous page