Xenbase Image ID: 44425

Fig. 2. IGF-I and Otx2 specifically block expression of Xbra and Xwnt-11 in vivo. Embryos at 4-cell stage were injected with synthetic mRNAs corresponding to IGF-I and Otx2 in the two dorsal blastomeres near the equatorial region. They were cultured to early gastrula (A–L) or late gastrula (M–R) stage for whole-mount in situ hybridization. (A) Xbra is expressed in the entire marginal zone mesoderm in the control embryo. (B) Dorsal injection of IGF-I blocked Xbra expression near injection sites. (C) Dorsal overexpression of Otx2 also blocked Xbra expression. Coinjection with Lac Z mRNA (red-stained cells) shows cell autonomous inhibtion of Xbra expression. (D) Xwnt-11 is expressed in the entire marginal zone in a control early gastrula. (E) Dorsal injection of IGF-I blocks Xwnt-11 expression. (F) Overexpression of Otx2 also blocked Xwnt-11 expression (red staining indicates cells coinjected with Lac Z and Otx2 mRNAs). (G–L) Dorsal injection of IGF-I or Otx2 had no effect on chordin (G–I) and Xnot (J–L) expression. (M–O) At late gastrula stage, the notochordal expression of Xbra was blocked by overexpression of IGF-I and Otx2. (P–R) Overexpression of IGF-I and Otx2 does not block Xnot expression at late gastrula stage, but prevents elongation of notochord and results in delayed gastrulation. Image published in: Carron C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 10.25 marginal zone tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Sometime during NF stage 11.5 to NF stage 12.5 notochord wnt11 Wnt11-R, WNT11-related, wnt11a, Wnt11r Xenopus Throughout NF stage 10.25 marginal zone chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 10.25 dorsal marginal zone not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot Xenopus Throughout NF stage 10.25 dorsal marginal zone not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot Xenopus Sometime during NF stage 11.5 to NF stage 12.5 dorsal marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + igf1 + NF10.5 (in situ hybridization) fig.2.b Expression Phenotype decreased amount tbxt.S expression in upper blastopore lip Xla Wt + otx2 + NF10.5 (in situ hybridization) fig.2.c Expression Phenotype decreased amount tbxt.S expression in upper blastopore lip Xla Wt + igf1 + NF10.5 (in situ hybridization) fig.2.e Expression Phenotype decreased amount wnt11b.L expression in upper blastopore lip Xla Wt + otx2 + NF10.5 (in situ hybridization) fig.2.f Expression Phenotype decreased amount wnt11b.L expression in upper blastopore lip Xla Wt + igf1 + NF12.5 (in situ hybridization) fig.2.n Anatomical Phenotype decreased size of the notochord Expression Phenotype decreased amount tbxt.S expression in notochord Xla Wt + igf1 + NF12.5 (morphology) fig.2.n Anatomical Phenotype abnormal gastrulation Xla Wt + otx2 + NF12.5 (in situ hybridization) fig.2.o Anatomical Phenotype decreased size of the notochord Expression Phenotype decreased amount tbxt.S expression in notochord Xla Wt + otx2 + NF12.5 (morphology) fig.2.o Anatomical Phenotype abnormal gastrulation Xla Wt + igf1 + NF12.5 (in situ hybridization) fig.2.q Anatomical Phenotype decreased size of the notochord Xla Wt + igf1 + NF12.5 (morphology) fig.2.q Anatomical Phenotype abnormal gastrulation Xla Wt + otx2 + NF12.5 (in situ hybridization) fig.2.r Anatomical Phenotype decreased size of the notochord Xla Wt + otx2 + NF12.5 (morphology) fig.2.r Anatomical Phenotype abnormal gastrulation Larger Image Printer Friendly View

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