Xenbase Image ID: 44437

Fig. 1. Expression pattern and antisense morpholino of Xenopus POSH (xPOSH). (A) RT-PCR analysis of temporal expression of POSH. Developmental stages are shown above each lane. Ornithine decarboxylase (ODC) was used as a loading control. (B) In situ hybridization analysis of spatial expression of POSH during early Xenopus development. (C) Scheme of the myc-tagged 5′-UTR POSH construct, the myc-tagged ORF POSH construct, and the morpholino sequence targeting the POSH 5′-UTR sequences. (D) xPOSH MO specifically inhibits the translation of its cognate mRNA. 5′-UTR (500 pg) and ORF (500 pg) xPOSH-Myc mRNA were injected with xPOSH MO (20 ng) or control MO (20 ng) into the animal region of embryos at the four-cell stage. Animal cap explants isolated at early gastrula stages were then subjected to immunoblot (IB) analysis with anti-Myc antibodies. Actin served as a specificity control. Image published in: Kim GH et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sh3rf1.S posh, rnf142, sh3md2, xPOSH X. laevis Throughout NF stage 3 (4-cell) animal hemisphere sh3rf1.S posh, rnf142, sh3md2, xPOSH X. laevis Throughout NF stage 10 to NF stage 12.5 marginal zone animal hemisphere sh3rf1.S posh, rnf142, sh3md2, xPOSH X. laevis Throughout NF stage 13 to NF stage 21 dorsal sh3rf1.S posh, rnf142, sh3md2, xPOSH X. laevis Sometime during NF stage 22 to NF stage 66 notochord otic vesicle pronephric kidney somite cement gland midbrain hindbrain telencephalon spinal cord branchial arch Image source: Published Larger Image Printer Friendly View

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