Xenbase Image ID: 44440

Fig. 4. POSH regulates Xenopus anterior neural development through JNK signaling pathway. (A–D) Two blastomeres of four-cell stage embryos were injected at their dorsal region with the indicated mRNAs. (A) Coinjection of xPOSH MO (20 ng) with XeJNK1 rescued the phenotypic defects of xPOSH MO in a dose-dependent manner. In addition, coinjection of JNK1 and CO MO did not exhibit the anterior neural defects. These phenotypes were classified into four classes: Index I, normal head and eye; Index II, weak head defect with small size eye; Index III, strong head defect with pin-shaped eye; Index IV, complete defect with no eye. (B–E) Whole-mount in situ hybridization with Otx2, En2, and Krox20 as probes. The reduction of anterior neural markers caused by xPOSH MO (20 ng; C) was reversed by XeJNK1 mRNA (2 ng; D). Coinjection of JNK1 and CO MO did not alter the expression of anterior neural genes (E). Image published in: Kim GH et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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