Xenbase Image ID: 44635

Fig. 3. TBX5 and TBX20 morphants fail to undergo looping and chamber formation and display reduced cardiac cell numbers. Cryosections of TBX5 and TBX20 morphant hearts taken at the anterior (outflow), middle (ventricular) and posterior (atrial) regions. (A-D) ControlMO, (E-H) TBX5MO and (I-L) TBX20MO. Sections stained for tropomyosin (red), DAPI (blue) and cardiac actin (green). (D,H,L) Same sections as B, F and J stained with cardiac actin. In the looped control heart, the middle ventricular section also contains the atrium. (M) Mean number of cells per heart obtained by cell counts of heart tissue in serial sections derived from a minimum of three embryos. Image published in: Brown DD et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue actc1.L actc, actna1, cardiac actin, cardiac alpha actin, muscle actin X. laevis Throughout NF stage 37 and 38 heart tpm1.L alpha-tm, alpha-tropomyosin, CH1 X. laevis Throughout NF stage 37 and 38 heart myocardium Image source: Published Larger Image Printer Friendly View

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