Xenbase Image ID: 44808

Figure 4. Zic1 regulates Xfeb expression in whole embryos. A,B: Zic1 induces ectopic Xfeb expression. Two-cell albino embryos were injected into one cell with 100 pg zic1 Delta C RNA and 25 pg lacZ RNA tracer. Embryos were fixed at stage 17 and stained for beta -galactosidase (blue) and Xfeb (brown). Ectopic Xfeb expression (white arrowheads) is induced by zic1 Delta C in the neural plate (A) and in non-neural tissues (B). C: A dominant interfering construct of Zic1 (dnzic1; Merzdorf and Sive, [2006]) inhibits endogenous Xfeb expression; 100 pg dnzic1 (together with 25 pg lacZ RNA tracer) inhibited endogenous Xfeb expression on the injected side in stage-14 embryos. Asterisks mark the posterior ends of the embryos. In all embryos, the injected sides are on the left. Black arrowheads mark the endogenous Xfeb expression domains. Image published in: Li S et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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