Xenbase Image ID: 44841

Fig. 7. XTcf-4A and XTcf-4C differ in restoring midbrain and isthmus defects. (A) In situ hybridizations of stage 30 embryos for marker gene expression specific for midbrain (wnt-2b, pax-6) or isthmus (en-2, wnt-1) demonstrate that the individual isoforms of XTcf-4 restore different regions of the brain. Dorsal view of wnt-2b, pax-6, and en-2 stained embryos reveal that the isoform XTcf-4A restored the expression of midbrain markers (wnt-2b and pax-6), whereas XTcf-4C restored the expression of the isthmus marker en-2. Transversal sections through the isthmus (row 4) demonstrate that en-2 expression was restored by XTcf-4C but not by XTcf-4A in XTCF-4-depleted embryos. The curved expression domain of wnt 1 posteriorly demarcating the isthmus (arrowhead) is best seen in the lateral view (see uninjected side, row 5). At the injected side (row 6), this curved wnt-1 expression was restored by XTcf 4C, but not by XTcf 4A. One blastomere of two-cell stage embryos was coinjected with EGFP and the indicated construct. At late neurula stages, embryos were sorted by the fluorescence of EGFP. The asterisks mark the injected side. m: midbrain, h: hindbrain. (B) Quantification of the ability of the XTcf-4 isoforms to restore the expression of wnt-2b, pax-6, en-2, fgf-8, and wnt-1 in XTcf-4-depleted embryos. The number of analyzed embryos is given. Image published in: Kunz M et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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