XB-IMG-44922
Xenbase Image ID: 44922
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Fig. 1. Expression of brunol1 in developing endoderm. (A) Expression of brunol1 in tadpoles at NF32, NF35 and NF39. A few scattered cells expressing brunol1 are detected at stage 32 in the extreme dorsal endoderm. By stage 35 expression can now be detected in more lateral endoderm. (D) NF41 isolated pancreas/liver tissue. At this early stage, brunol1 is expressed in a punctate pattern only in the dorsal pancreas. (E) NF44 isolated pancreas/liver. Expression of brunol1 is now detected throughout the entire pancreas. (F) Double in situ hybridization of brunol1 (purple, arrow) and insulin (red, arrowhead) in NF44 isolated pancreas/liver. No overlap in expression is seen. Insulin expression is confined to the dorsal pancreas, whereas brunol1 expression is also present in the ventral part of the pancreas. (G,H) NF41 and NF44 isolated whole gut. Expression is punctate throughout the entire gastrointestinal tract. (I) Double in situ hybridization of isolated whole gut for chromogranin A (red, arrowhead) and brunol1 (purple, arrow). No overlap in expression is seen. Chromogranin A is expressed in all differentiated endocrine cells. (J) pH3 staining at NF32. Expression in the endoderm is only detected in scattered cells (arrowhead). Mesodermal expression is also detected. (K) pH3 staining at NF39. Proliferating cells are found throughout the entire endoderm, similar to brunol1. (L) pH3 staining at NF40 in isolated liver/pancreas tissue. Proliferating cells are only found in the dorsal pancreas (line demarcates dorsal versus ventral portions of the pancreas). Proliferating cells are also found in the liver.
Image published in: Horb LD and Horb ME (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V.
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