Xenbase Image ID: 45195

Fig. 2. Knockdown of wt1 results in a glomus phenotype. (A) Sequences of the two pseudo-alleles of Xenopus wt1 and the location of wt1-MO1 and wt1-MO2. Mismatches between wt1-MO and the second allele of wt1 are indicated in red. The translational start site is boxed. (B) Schematic of the wt1-GFP reporter constructs. (C-E) Fluorescence of uninjected control embryos (C,D), embryos injected with the wt1-GFP reporter mRNA (C′), with wt1-MO1+2 and the wt1-GFP reporter (C′), wt1(mut)-GFP (D′) or with wt1-MO1+2 and the wt1(mut)-GFP (D′) at stage 10. The results of multiple experiments were quantified (E); the number of embryos analyzed is indicated above the bars. (F-K) Phenotype, histology and β1-Integrin immunofluorescence of wt1-MO1+2-injected embryos (G,I,K) and sibling controls (F,H,J) at stage 40 (F,G) and stage 42 (H-K). Red arrowheads indicate glomus in H. Image published in: White JT et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wt1.S wt-1, wt1-a, wt1-b, Xwt-1, xwt1 X. laevis Throughout NF stage 40 to NF stage 42 podocyte glomus pronephric kidney Image source: Published Larger Image Printer Friendly View

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