Xenbase Image ID: 45235

Fig. 6. Xlim1 is regulated by miR-30a-5p. (A-D′) Whole-mount in situ hybridization for Pax2 (A,A′), Pax8 (B,B′), Hnf1-β (C,C′) and Xlim1 (D,D′), comparing uninjected and miR-30a5p-MO-injected Xenopus embryos at stage 39. (E-G′) Whole-mount in situ hybridization for Xlim1 mRNA of uninjected and miR-30a5p-MO-injected Xenopus embryos at stages 31, 35 and 39. Note that Xlim1 expression is maintained at a higher level in miR-30a5p-MO-injected embryos after stage 35, when endogenous expression of miR-30a-5p can first be detected (arrows in D′,G′). (H,H′) Immunohistochemistry with 4A6 of stage 40 embryos injected with 4 pg pCS2-Lhx1* DNA into one blastomere at the 4-cell stage, comparing the left with the right side. Arrows indicate changes in 4A6 staining. Ectopic expression of Lhx1 mRNA transcribed from the pCS2-Lhx1* was confirmed by in situ hybridization (data not shown). Image published in: Agrawal R et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 39 pronephric kidney pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 39 pronephric kidney hnf1b.L hnf-1beta, hnf1-beta, hnf1beta, lfb3, tcf2, vhnf1 X. laevis Throughout NF stage 39 pronephric kidney lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 31 to NF stage 39 pronephric kidney Image source: Published Larger Image Printer Friendly View

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